ABSTRACT: "Novel Polymer Particles and Their Applications in Biotechnology"
Microfluidics is the science of controlled droplet formation in micro-channels. The simplest microfluidic setup is based on tubing, needles, syringes and syringe pumps. Utilizing this setup, we synthesized a whole new set of complex particles that vary both physically (shape) and chemically (composition and reactivity).
First of all we made porous beads that were turned into Janus beads via "sandwich" microcontact printing: A monolayer of reactive beads are sandwiched between two silicone stamps, which transfer two different chemicals covalently onto opposing faces of beads. We also introduced HIPE droplets-in-microfluidics approach, which is forming reactive emulsion droplets in a channel.
Polymerization of these droplets yielded beads, capsules, rods and even liquid marble forming particles. In addition, we constructed beads from thiol-ene and thiol-yne monomers as an alternative to vinyl polymerization. Obtained thiol and alkyne functionalized beads allowed us to conduct numerous click-type reactions in solid phase.
Finally I will also explain the seeded suspension polymerization method that is the proprietary technology of Life Technologies for making monodisperse, micron sized, magnetic polymer beads. Cell isolation, in-vitro diagnostics and DNA sequencing are among the applications of our beads.