Title: Nanostencil Lithography for Plasmonics and Applications on Systems Immunology
Speaker: Dr. Serap Aksu (ETH-Zuerich)
Date/Time: Wednesday 24/12/14 at 10:00
Place: SUNUM G111
Development of low cost nanolithography tools generating high resolution nanostructures in a highthroughput fashion is crucial for transferring next generation biophotonic technologies in daily life. This talk will first introduce a patterning technique, Nanostencil Lithography (NSL), for fabrication of engineered metallic plasmonic nano-antennas and metamaterials operating at visible and infrared wavelengths. NSL enables fabrication of precisely engineered antenna arrays with optical qualities comparable to the ones fabricated by standard electron beam lithography. Furthermore, we demonstrate that NSL has the flexibility to create complex plasmonic nanostructure designs in precise arrays on the substrates that are difficult to work with e-beam and ion beam lithography tools (i.e. polymers). Mechanically tunable flexible optical materials and spectroscopy probes integrated on curved surfaces could be fabricated at 10 nm accuracy. As a versatile nanopatterning tool, NSL also has capability to pattern bioprobes with nanoscale features on biocompatible polymer substrate. Finally we present the first application of NSL fabricated low cost infrared nanoantenna arrays for plasmonically enhanced vibrational biospectroscopy. Using nanoantenna arrays fabricated with reusable stencils we demonstrate the detection of immunologically important protein monolayers with thickness as small as 3 nm, and perform antibody assays.
Second part of the talk will introduce an optofluidic platform for time dependent investigation of single immune cells. Quantitative analysis of dynamic input-output relationships in single-cells is crucial for building models of immune system. For high-throughput quantitative analysis we developed an automated microfluidic system that controllably delivers stimulants to individual cells and simultaneously measures the cell response. The proposed system can both employ nano-liter immunoassays or label-free localized surface plasmon resonance (LSPR) readout. PDMS membranevalve based fluid control, isolated nano-liter sized chambers and video microscopy allows thousands of quantitative measurements. This system greatly improves the functionality and precision of single-cell analysis, and allows label free, in-situ, real-time investigation.
Contact: Cleva Ow Yang