MSc. Thesis Defense: Ebru Uysal
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Impacts of Single-Walled Carbon Nanotubes on Polymerase Chain Reaction

 

Ebru UYSAL
Biology and Bioengineering, MSc. Thesis, 2014

 

Thesis Jury

Prof. Dr. Hikmet BUDAK

Assoc.Prof. Levent ÖZTÜRK

Prof. Dr. Ersin GÖĞÜŞ

Prof. Dr. Hüveyda BAŞAĞA

 

 

Date &Time: 25th, 2014 –  13.30

Place: FENS 1040

Keywords : Polymerase Chain Reaction, nonoPCR, Single-Walled Carbon Nanotubes

 

Abstract

 

Recently, the Polymerase Chain Reaction technique has begun to benefit from nanotechnology. In this paper, effects of single-walled carbon nanotubes in the Polymerase Chain Reaction were investigated by Polyacyrlamide Gel Electrophoresis, Dynamic Light Scattering Techniques and Scanning Electron Microscopy. The unique ability to amplify low copy number DNA in a short time period has made in vitro Polymerase Chain Reaction (PCR) one of the most crucial techniques in biological studies and medical. In order to utilize this technique with its full potential, certain obstacles such as nonspecific by-products, low yield, and complexity of GC rich and long genomic DNA amplification need to be eliminated.

 

Nanomaterial-assisted PCR is termed as nanoPCR, is a new flourishing area in biotechnology that combined the nanostructured materials with PCR reaction to obtain improved and qualified results. Nanomaterials have unique physical and chemical properties, such as high thermal conductivity, stability and high surface to volume ratios that makes them significant for numerous of research aread. The effects of nanomaterials in PCR depend on their size, shape, concentration, heat conductivity, electron transfer properties and surface modifications. Carbon nanotubes have hydrophobic surface area that has the tendency to agglomerate and minimize the energy of surface and interaction with the environment. In this paper, we demonstrate the impact of single-walled carbon nanotubes in PCR. In order to, eliminate large agglomerates sonicated, centrifuged and filtered single-walled carbon nanotubes are used for experiments. The sonicated single-walled carbon nanotubes are long and have large aggregates. In comparison to sonicated single-walled carbon nanotubes, centrifuged and filtered ones are smaller and short. In order to measure the relative hydrodynamic size of sonicated, centrifuged and filtered single-walled carbon nanotubes, Dynamic Light Scattering (DLS) is used. According to the results, PCR enhancement that can be observed by intensities of electrophoretic bands of target DNA is affected by single-walled carbon nanotubes at different range of concentrations and the relative hydrodynamic sizes. This work could be convenient for the further studies that are focused on exploitation of single-walled carbon nanotubes for nanobiology, nanomedicine and biosensing.