DESIGN AND DEVELOPMENT OF A MICROFLUIDIC DEVICE TO MONITOR IRON BINDING DYNAMICS TO IRON TRANSPORT PROTEINS
Material Science and Engineering, MSc. Thesis Defense, 2015
Prof. Dr. Canan ATILGAN (Thesis Advisor),
Prof. Dr. Ali Rana ATILGAN, Prof. Dr. Ali KOŞAR,
Asst. Prof. Dr. Alpay TARALP, Assoc. Prof. Dr. Elif ÖZKIRIMLI ÖLMEZ
Date & Time: 29th, 2015 – 11 AM
Keywords: Microfluidic systems, ferric binding protein, iron binding dynamics
Iron binding mechanisms of proteins are riveting studies because of the great importance of iron molecules for the metabolism of humans and other organisms. In this dissertation, a microfluidic device was designed and developed to measure iron binding constants to ferric binding proteins in humans (Transferrin) and bacteria (Haemophilus Influenzae Ferric Binding Protein (FBP)), to assess the effect of different environmental conditions on the kinetics of iron – protein association. Firstly, a microfluidic chip housing an effective mixing component was designed and fabricated by using PMMA and PDMS as a material. Both designs were tested by using bromocresol – acetic acid reactions, where the color change from blue to green and from green to yellow can be observed by lowering the pH. The reaction was monitored with high resolution camera and the color change was analyzed to determine the mixing efficiency. Using ANSYS Fluent software these geometries were modified and improved designs were suggested. Because the FBP is not commercially available, it was expressed and purified by using recombinant DNA technology for monitoring iron binding dynamics in the microfluidic device as future work.