Title: Remodeling of Pancreatic Cystic Fibrosis using Human Pluripotent Stem Cell Derived Pancreatic Duct Epithelial Cells
Speaker: Senem Simsek
Date/Time: 25/02/2015, 12:40 - 13:30
Place: FENS G035
Abstract: Pancreatic cystic fibrosis (CF) is an inherited physiological disorder characterized with either impairment or insufficiency of electrolyte transport between the apical membrane of pancreatic duct epithelium cells (PDECs) and pancreatic juice. Cystic fibrosis conducting regulator (CFTR) functions as cAMP regulated weak field anion conductance channel on the apical side of PDECs. The most common CFTR mutation F508 is followed by frame shift and splicing error mutations. They contribute the pathophysiology of the disease via distrupting CFTR function in different spectrum. Recent studies have reported lung CF human pluripotent cell and human CF intestine organoids. Here, we model CFTR maturation and mislocalization errors in pancreatic CF epithelial cells via in vitro directed differentiation of CF-hiPSCs (human induced pluripotent stem cells) through a unique genetic based approach functionality test of in vivo anion conductance. We applied our concept as a supporter on pharmacologically repaired CFPAC1 cell line and PDECs carrying F508 mutation.
Bio: Dr. Simsek has a proven track record, high responsibility and integrity and commitment in human pluripotent stem cell (hESC/hiPSC) research, disease modeling and drug therapy in Weill Cornell Medical College. Her major research interest is to manipulate pluripotent stem cell fate using chemical and biological approaches for generation of lineage committed cells and to implement these protocols to 3D bio-printing tools for the establishment of complex disease modeling, drug discovery and future cell therapy platforms.