Faculty of Engineering and Natural Sciences
A QUANTUM OF VIBRATION IN AN UNEXPECTED PLACE
X-ray scattering and its biological applications (*)
Esen Ercan Alp
Advanced Photon Source, Argonne National Laboratory, Argonne Illinois 60439
& UNAM, Bilkent University, Ankara, Turkey
Modern X-ray scattering techniques as applied to Biology cover a large frontier from protein crystallography to small angle x-ray scattering, picosecond time-resolved diffraction to inelastic x-ray scattering, and diffraction-enhanced imaging. Progress in x-rays and synchrotron radiation had significant influence on the development of modern biology, starting with the discovery of atomic structure of DNA in 1953, and lately in Kornberg’s discovery of the transcription process for making mRNA, in both cases resulting in Nobel Prizes. In this presentation, I would like to emphasize the role of synchrotron radiation and present our own work of using inelastic x-ray scattering with resolution power exceeding 107-1012 to study atomic and collective behavior of atoms, molecules, model compounds like porphyrins, lipids, proteins and enzymes.
Some of our recent publications:
1. D. Liu, et al, “Studies of Phononlike Low-Energy Excitations of Protein Molecules”, Physical Review Letters, 101 (2008) 135501
2.Y. Guo, et al, “Characterization of the Fe Site in Iron-Sulfur Cluster-Free Hydrogenase (Hmd) and of a Model Compound via Nuclear Resonance Vibrational Spectroscopy (NRVS)” Inorganic Chemistry, 47 (2008) 3969
3. V. Stravoitova, et al, “Intermolecular Dynamics in Crystalline Iron Octaethylporphyrin (FeOEP)”, J. Phys. Chem. B 112 (2008) 12656
4.Y. M. Xiao, et al, “Dynamics of Rhodobacter capsulatus [2Fe-2S] Ferredoxin via Nuclear Resonant Vibrational Spectroscopy (NRVS) and Resonance Raman Spectroscopy”, Biochemistry, 47 (2008) 6612-6627
5.Y.S. Guo, et al, “Characterization of the Fe Site in Methanothermobacter marburgensis Hydrogenase (mHmd) via Nuclear Resonance Vibrational Spectroscopy (NRVS)”, Inorg. Chem.,2008, 47, 3969-3977
6. N. Silvernail, et al, “Interplay of Structure and Vibrational Dynamics in Six-Coordinate Heme Nitrosyls” Journal of Americal Chemical Society, 129 (2007) 2200.
7. B. Leu, et al, ”Quantitative Vibrational Dynamics of Iron in Carbonyl Porphyrins”, Biophysics Journal, 92 (2007) 3764-3783.
8. Y. Xiao, et al, “How Nitrogenase Shakes - Initial Information about P-Cluster and FeMo-cofactor
Normal Modes from Nuclear Resonance Vibrational Spectroscopy (NRVS)”, Journal of Americal Chemical Society, 128 (2006) 7608.
9. Y. Liu, et al, “Effects of Counterion Valency on the Damping of Phonons Propagating along the Axial Direction of Liquid Crystalline DNA,” J. Chem. Phys. 123, (2005), 214909
(*) Work performed in collaboration with W. Sturhahn, H. Sinn, T. Toellner, J. Zhao, A. Alatas, A. Said, and H. Yavas, all of Argonne National Laboratory, S. H. Chen of MIT, T. Sage of
University, R. Scheidt of University of Notre Dame and S. Durbin of
University. This work is supported by US DOE-BES Materials Science under contract number W-31-109-ENG-38.
October 21, 2008, 11:40, FENS L035