Biological Sciences - Bioengineering Seminar
  • FENS
  • Biological Sciences - Bioengineering Seminar

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Cömert Kural, Harvard Medical School
13:40, May 18, 2011
FENS 1040

Clathrin mediated endocytosis is major cellular uptake mechanism. So far, real‐time
observation of this phenomenon has been achieved using methods that are limited to two
dimensional spatial analysis even though cells have three dimensional geometries. Here we
have used a fast, confocal based, three dimensional automated tracking scheme to analyze
clathrin coat dynamics at the ventral and dorsal surfaces of mammalian cells. This technique
presents a direct observation of a clathrin mediated virus entry at the apical surface of
polarized cells for the first time. Our analysis has shown that a sub‐population of clathrincoated
structures ascend from plasma membrane for hundreds of nanometers before they
uncoat. Elevation of these structures depends on actin machinery and is enhanced by
increasing membrane tension. On the migrating cells, we have found that clathrin‐mediated
endocytosis is constrained to the dorsal side of the plasma membrane at the sites of protrusion.
Clathrin‐coats emanating at the leading edge move towards the center of the cell with
velocities inversely proportional to the migration speed of the cell.
Dr. Kural received his BS degree in Physics from Bilkent University, and his PhD in Biophysics and Computational
Biology from University of Illinois at Urbana‐Champaign. He is currently working as a Helen Hay Whitney Fellow in
Kirchhausen Lab.