Advanced FIB / FEB Dual Beam Prototyping
Real Time AFM in Biology
Dr. Harald Plank
Department Head for FIB and AFM
Institute for Electron Microscopy and Fine Structure Research (FELMI)
Graz University of Technology
The presentation gives an overview about the scientific main activities of the “FIB and AFM Department” at the “Institute for Electron Microscopy” (Graz University of Technology; Austria): 1) fabrication of functional nanostructures via focused electron beams, 2) soft matter processing via focused ion beams, and 3) real time atomic force microscopy of enzymatic cellulose degradation.
The first part of this talk focuses on electron beam induced deposition (EBID) from the gas phase which allows for the fabrication of 3-dimensional, functional nanostructures (conductive, insulating, magnetic, …). The presentation gives an overview about fundamental processes and demonstrates how preparation parameters can be used to improve deposition performance, deposit functionality, and spatial resolution which have been the limiting elements in the past.
In the second part, emphasis is put on the increasing demand for nanostructuring tools in combination with low melting materials (polymers, biological materials, …) for prototyping applications such as stamp fabrication or functional morphology tuning. Focused ion beams (FIB) are highly suited for such purposes, however, show often a strongly degrading character due to the high temperatures during processing. As revealed in this overview, these high temperatures are not only caused by the intrinsic ion-matter interaction but also due to the standard process procedures itself. To eliminate this technically induced heating, alternative patterning strategies are introduced which considerably reduce the thermal stress for the sample and successfully decrease morphological and chemical degradation.
The last part of the presentation is focused on real time atomic force microscopy (AFM) investigations of biological systems in liquids. Nanoflat cellulose substrates have been prepared via special preparation methods revealing crystalline areas embedded in more amorphous regions. Dynamic AFM experiments during incubation with the supernatant of the Trichoderme reesei as well as with their isolated components give a comprehensive insight in individual processes and synergistic effects which have never been observed directly in the past.
November 30, 2011, 14:30, FENS G032