Genomics for lunch: physical mapping of wheat chromosome 1AL
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BIO551-201101:  Biology  seminar: December  7, 2011
Location: FENS L065
Time: 13:40

Genomics for lunch: physical mapping of wheat chromosome 1AL

Genomics for lunch: physical mapping of wheat chromosome 1AL

Bread wheat (Triticum aestivum L.) is one of the most important crops
globally and a high priority for genetic improvement, but its large
and complex genome has been seen as intractable to whole genome
sequencing.  Isolation of individual wheat chromosome arms is now
enabling large-scale analysis.  Greater understanding of individual
wheat chromosomes is necessary, to facilitate breeding for wheat
improvement and assembly of the entire genome sequence.
As part of the EU-FP7 funded Trıticeae genome project consortium, we
have constructed BAC libraries from the long arm of T. aestivum
chromosome 1A (1AL).  The BACs were fingerprinted and used to
construct a physical map of the chromosome, along with a Minimal
Tiling Path (MTP) of clones covering the chromosome arm.  The ends of
the 7470 BAC clones in the MTP were sequenced and used to survey the
content of the chromosome, as well as design markers.  A total of 366
markers have been placed on the physical map;  ongoing analysis of the
chromosome and mapping approaches will be presented.