Revealing the Extracellular Interactome: Cell Surface Receptor Function through Protein Interaction Networks
Engin Ozkan, Ph.D.
Time: 22 Aralık 13:40; Place: FENS 2008
Homophilic and heterophilic interactions between extracellular proteins mediate essential developmental processes in animals, and are crucial to the functioning of neural and immune systems. The network of pairwise interactions that underlie these important functions remain largely unknown. We took a biochemical approach to carry out a complete pairwise interaction screen for 202 gene products from Drosophila melanogaster, covering 20,503 potential interactions. We validated a subset of these interactions via independent methods, such as surface plasmon resonance, and tissue staining. We further studied the multi-functional cell adhesion molecule family of Syg-1, Syg-2 and their homologues. We determined several crystal structures of proteins and their complexes from this family, revealing the molecular basis of cell adhesion in Syg-mediated synaptogenesis, axon guidance, muscle cell fusion, and the formation of the kidney filtration barrier.