DEVELOPMENT OF SPION-BASED THERANOSTIC NANOCARRIERS FOR SMALL RNA DELIVERY AND CANCER GENE THERAPY
Molecular Biology, Genetics and Bioengineering, MSc. Thesis, 2016
Assoc. Prof. Devrim Gozuacik (Thesis Advisor), Assoc. Prof. Havva Funda Yagci Acar,
Prof. Dr. Ali Koşar
Date & Time: August4th, 2016 – 10:30AM
Place: FENS G025
Keywords : Nanomedicine, nanoparticle-based gene therapy, microRNA, cancer
Nanoparticles can be used for medical purposes, including diagnosis, therapy and follow-up of diseases. In recent years, small RNAs including microRNAs have emerged as novel diagnostic and therapeutic tools in the field of cancer research. Combination of nanoparticles with small RNAs has the potential to open new avenues for targeted gene therapy of diseases including, cancer.
In this thesis, multifunctional and biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) were created and tested for gene therapy purposes. Nanoparticles were covered with polymers, coated with RNA binding (RB) proteins and antibodies in order to target and kill tumor cells. MicroRNA-based patented drugs were utilized as payload. Components that were used to create multifunctional and biocompatible SPIONs were strongly binding RNAs, increasing their stability, specifically recognizing HER2+ breast cancer cells, and effectively modulating autophagy. Moreover, to facilitate tracing, fluorescent probes were added to nanoparticles. Magnetic manipulation tests were also performed.
These novel theranostic, multifunctional and cancer-targeting nanoparticles may potentially be used alone or in combination with chemotherapy drugs for cancer treatment purposes. Exploitation of these particles for the diagnosis and hyperthermia treatment of primary and metastatic tumors is also possible.