SEMINAR:Behaviour of Neuro-2A Cells on Femtosecond Laser Micromachined..19-10-2020

Speaker:  Prof. Alpan Bek, Middle East Technical University

Title: Behaviour of Neuro-2A Cells on Femtosecond Laser Micromachined Silicon Substrates

Date/Time: 21 October 2020 13:40 - 2:30 pm   

Zoom: Meeting ID: 960 3228 8444 Passcode: Not required

Abstract: The interaction of neural cells with silicon surfaces is important for basic research as well as for various possible applications, such as silicon-based neural implants and neurochips. Laser structuring of silicon provides a quick and versatile method for the generation of complex, hierarchical topographies on precise locations of the substrate. The behaviour of Neuro-2A cells with laser-structured silicon substrates was studied using a live-imaging setup with fluorescence microscopy. Neuro-2A cells were able to adhere to polished silicon, ripples and microcolumns to different extents, depending on the substrate topography and incubation time. Initially, cells adhere much better to structured areas, resulting in visible cell patterning on the substrates. Time-lapse microscopy revealed cell exploration and motility behaviours on the substrates. Cell motility was significantly decreased on structured substrates, with whole area microcolumns having the slowest cell motility. On polished silicon, cells were found to interact with the substrates using lamellipodia and filopodia. After 24 or 48 hours, cells were better able to adhere to polished as well as structured silicon.  On the one hand, neurite alignment was observed on microcolumn and trench substrates. On the other hand, highly processed substrates were found to be inhibitory to cell growth and resulted in poor cell health.

Bio: Alpan Bek has completed his BSc and MSc studies at Bilkent University Department of Phyics. He has taken part in experimental research on optical properties of low dimensional systems and integrated optics under supervision of Prof. Atilla Aydinli. He has obtained his PhD degree from Ecole Polytechnique Federale de Lausanne (EPFL) while he conducted his PhD work at the Max-Planck Institute for Solid State Research in Stuttgart, Germany under supervision of Prof. Klaus Kern. He has constructed an optical microscope that can resolve structures down to 5 nm with visible light. He has had a post-doctoral stay at Ludwig Maximillian's University of Munich (LMU) in the group of Prof. Jochen Feldmann and conducted research together with Prof. Thomas Klar on plasmonic systems. Afterwards he has moved to Cluster in Biomedicine (CBM) in Trieste, Italy to work together with Dr. Marco Lazzarino on tip enhanced Raman spectroscopy (TERS). In 2011 he has returned back to Turkey as a faculty member in Physics Department of Middle East Technical University. He is currently conducting research in the field of nanophotonics as the leader of the Nano-Optics Research Group and as a member of The Center for Solar Energy Research and Applications (GÜNAM).