BIO 580- Special Topics in Biological Sciences and Bioengineering "BIOSENSORS"
Sabanci University
Faculty of Engineering and Natural Sciences
10834 - BIO 580 Special Topics in Biological Sciences and Bioengineering
"BIOSENSORS"
Fall 2009‐2010
(2) Lehninger Principles of Biochemistry, Fourth Edition/ David L. Nelson and
Michael M. Cox
(3) Smart biosensor technology / edited by George K. Knopf, Amarjeet S. Bassi.
R857.B54 S66 2007
(4) Other textbooks shall also be used as references.
Recitation hours : Friday 16:40‐17:30
tools employed in biophysical methods, biomolecular technology and integrating electronic signal transducers. Studies pertaining how engineering and biological approaches can be combined to solve problems in diagnosis of diseases, such as diabetes, cancer or detection of other analytes/biomarkers – emphasizing integration of biological macromolecules (DNA, enzymes, and antibodies) and methodologies for the development of biosensors. Emphasis will also be given to molecular mechanisms underlying cellular processes and
their integration with electronic transducers for biosensor applications.
Materials Science, Chemistry and Electronics
Grading Policy : Midterm 1‐25% Midterm 2‐25% Final‐ 35% Homework/Seminar – 15%
History of biosensor development, applications and requirements of biosensors and classification
Principles of molecular recognition and transduction signal acquisition
o Sources of Biological Recognition elements – enzymes/proteins, ssDNAs, antibody and others
o Design considerations for use of recognition elements in biosensors
o Modeling of reactions for various biosensor applications‐ electrochemical, optical,
piezoelectric, colorimetric, fluorometric and others.
Modification of sensor surfaces and immobilization techniqueso
Covalent modification of surfaces using surface chemistry
o Self Assembled Monolayers (SAM) and adsorptions
o Other ways to immobilize biological macromolecules on various solid surfaces
Detection methods and Physical Sensors
o Electrodes/transducers – electrochemical (amperometric, potentiometric, and
conductimetric transductions)
o Other sensors ‐ for e.g., optical sensors (colorimetric/fluorimetric/luminometric sensors),
Surface Plasmon Resonance (SPR) sensors, and piezoelectric resonators.
Fabrication of biosensors
o Miniaturization‐application of nano‐materials, nanoparticles, carbon nanotubes (CNTs) and
others
o Biocompatibility – stability, reproducibility and repeatability of biomolecules on transducer
surfaces
Data acquisition, statistical and error analysis
o Inter and Intra‐assays and Coefficient of variation (CV)
o Signal to noise ratio
o Normalization/optimization and signal retrieval
Examples of commercial biosensors